Protective Role of Alternanthera Sessilis (Linn.) Silver Nanoparticles and Its Ethanolic Extract against Rotenone Induced Parkinsonism

Biosynthesis of nanoparticles incorporating the herbal extracts can potentially eliminate the toxicity problem. Green synthesis of silver nanoparticles was done by using the ethanolic extract of Alternanthera sessilis (Linn.) and Silver nitrate. The anti-parkinsonism activity of ethanolic extract of Alternanthera sessilis (Linn.) (EEAS) and its silver nanoparticles (ASAgNPs) was evaluated using oxidative stress model of Parkinsonism using Rotenone. Parkinsonism was induced by administration of Rotenone (12 mg/kg). The effect of EEAS (200 mg/kg) and ASAgNPs (20mg/kg) were assessed by antioxidant assays such as Glutathione and Lipid peroxidation. The behavioural parameters such as rearing, selfgrooming, ambulation activity and muscle rigidity were analysed using open field apparatus and bar test. EEAS and ASAgNPs caused significant neuroprotection as evidenced by decrease in catalepsy and muscle rigidity along with a significant increase in locomotion. The reduction in the lipid peroxidation and an increase in the GSH, indicate the reduction in oxidative stress in the brain of animals.